In 2000, Holloway married George "Jug" Twitty, an Alabama businessman, and moved with her children to Mountain Brook, Alabama.

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Twitty was criticized for her focus on Joran van der Sloot and the Kalpoe brothers, to the exclusion of any other theory as to what happened to Natalee.

According to the lawsuit filed by the Kalpoe brothers, she has (on various television programs) repeatedly accused them and van der Sloot of "sexual assault" and "gang rape" of her daughter.

She continued her studies at Arkansas State University in Jonesboro, where she received a master's degree in speech pathology.

After the couple divorced in 1993, she raised her two children on her own.

Twitty said that she vowed to share her story shortly following her daughter's disappearance, in the hope that other families would not suffer what she had to go through.

After searchers had spent five months in an unsuccessful quest to find Natalee, her attorney John Q.Van der Sloot initially denied knowing Natalee's name, but he then told the following story, with which Deepak Kalpoe, who was present, agreed: Holloway stated that she received copies of police statements stating that van der Sloot admitted having sex with Natalee at his home and described intimate details of her.She has never released copies of the alleged statement, though she characterizes them as admissions of "sexual assault" However, Vinda de Sousa, former Holloway/Twitty family attorney from Aruba understood that the admission may have been for consensual sex.Kelly said that he secretly went to Aruba in April to meet with Van der Sloot, who was desperate for money, and gave him 0.Kelly notified the Federal Bureau of Investigation to set up a sting operation with the Aruban authorities.Following the airing of the program, Beth Twitty, adhering to the position that the tapes represent the way events transpired, believed that Van der Sloot dumped Natalee's body, possibly alive, into the Caribbean.